The cycling conditions were 95C for 5 min; 95C for 30 sec, 52C for 1 min, and 65C for 2 min for 40 cycles; and an extension step at 65C for 5 min. samples, we used the affinity probe biotin-labeled HA, which only binds to CD44 in its active conformation [24]. The results demonstrated increased … Continue reading The cycling conditions were 95C for 5 min; 95C for 30 sec, 52C for 1 min, and 65C for 2 min for 40 cycles; and an extension step at 65C for 5 min